
Rennet is a crucial ingredient in cheesemaking, used to separate milk into curds and whey. It is a complex set of enzymes produced in the stomachs of ruminant mammals, with the key component being chymosin, a protease enzyme that curdles casein in milk. Traditionally, rennet was derived from the stomach lining of young ruminants, but today, most cheese is made using chymosin from bacterial sources. This shift has occurred due to the limited availability of mammalian stomachs and the desire to find alternatives that are less expensive and suitable for vegetarians and those keeping kosher. The use of rennet is essential for creating the desired texture and flavour profile in various cheeses, making it a key topic of interest in the world of cheesemaking.
| Characteristics | Values |
|---|---|
| What is rennet? | Rennet is a complex set of enzymes produced in the stomachs of ruminant mammals. |
| How is it used in cheese-making? | Rennet is used to separate milk into solid curds and liquid whey. |
| What does it contain? | Rennet contains enzymes such as chymosin, pepsin, and a lipase. |
| What is chymosin? | Chymosin is a protease enzyme that curdles the casein in milk. |
| What is pepsin? | Pepsin is a digestive enzyme that impacts the aging process of cheese. |
| What are the sources of rennet? | Sources of rennet include animals >(calves, lambs, goats), plants (thistle, cardoon, artichokes), and microbial/vegetarian rennet. |
| What are the benefits of different sources? | Animal rennet is traditional and has reliable results in yield and flavor. Plant rennet is bitter if aged too long. Microbial rennet is suitable for vegan and vegetarian cheeses. |
| What are the forms of rennet? | Rennet is available in liquid, paste, powder, and tablet forms. |
| How much rennet is used in cheese? | Typically, 1 kg of cheese contains about 0.0003 g of rennet enzymes. |
| What are substitutes for rennet? | Substitutes for rennet include acids (like vinegar), cultures, and enzymes from plants and fungi. |
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What You'll Learn
- Rennet is a complex set of enzymes produced in the stomachs of ruminant mammals
- Rennet separates milk into solid curds and liquid whey
- Rennet is added to milk in liquid, paste, powder or tablet form
- Rennet is crucial for finding the right texture for your cheese
- Rennet is used to make vegetarian and vegan cheese

Rennet is a complex set of enzymes produced in the stomachs of ruminant mammals
Rennet is traditionally sourced from the stomach lining of young ruminants, such as calves, lambs, and kids (young goats). It is produced naturally in their digestive systems to help curdle milk for easier digestion. However, as animals age and their diet shifts from milk to grass, the production of rennet decreases. Therefore, rennet for cheesemaking is typically derived from young animals, making it a byproduct of the beef industry.
The process of extracting rennet involves drying and cleaning the stomachs of these young animals, cutting them into small pieces, and soaking them in salt water or whey with vinegar or wine to lower the pH. The solution is then filtered, leaving crude rennet that can coagulate milk. This traditional method of rennet production is still practised in some places, but manufactured rennet products have gained preference due to their controlled potency and predictable results.
Over time, alternative sources of rennet have been explored due to the limited availability of mammalian stomachs and the desire for vegetarian and vegan options. Vegetable rennet, derived from plants like cardoon thistle, artichokes, nettles, and certain fungi, is a relatively new technique. It is often used in commercial cheesemaking and for producing vegetarian and vegan cheeses. However, vegetable rennet can introduce bitterness to the cheese if aged for extended periods.
Another alternative is microbial rennet, which is created in a lab and sometimes derived from mushrooms. It is composed of 100% chymosin, lacking the nuanced balance of enzymes found in animal rennet. While some consider it a GMO product, it is widely used in industrial cheesemaking due to its lower cost and suitability for various dietary preferences.
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Rennet separates milk into solid curds and liquid whey
Rennet is a complex set of enzymes produced in the stomachs of ruminant mammals. It is traditionally sourced from the stomach lining of young ruminants, but it can also be made using microbial, plant, or vegetable sources. The key component of rennet is chymosin, a protease enzyme that curdles the casein in milk. In addition to chymosin, rennet contains other enzymes, such as pepsin and a lipase.
When used in cheesemaking, rennet separates milk into solid curds and liquid whey. This process is known as coagulation. Specifically, rennet helps to extract the maximum amount of milk solids from the milk, allowing the curds of the cheese to form. The enzymes in rennet are activated when temperatures reach 85 to 105 degrees Fahrenheit, and they continue to aid in coagulation until the liquid reaches 140 degrees Fahrenheit. The separation of curds and whey is crucial in cheesemaking, as it affects the final taste and texture of the cheese. Different types of cheeses require different levels of firmness, which can be achieved through the use of rennet. For example, a soft Brie may have looser curds, while a hard Romano would benefit from firmer curds.
The traditional view is that plant and microbial rennet can result in bitterness and low yield in cheese, especially when aged for long periods. However, microbial coagulants have improved greatly over the years, and several high-quality cheeses can now be produced with microbial rennet. Vegetable rennet is a newer product and technique, and it is often used in store-bought cheeses. Animal rennet, on the other hand, has been a part of cheesemaking for centuries and is typically used in artisanal cheese production.
Today, animal rennet is mainly a byproduct of the beef industry, sourced from the stomachs of calves, lambs, or kids (young goats). Calf rennet is said to produce more reliable results in terms of yield and flavor profile, leading to less defective cheese. It is also a natural and non-GMO option. However, due to limited availability and ethical considerations, cheese makers have sought alternative sources of rennet.
With genetic engineering, it became possible to isolate rennet genes from animals and introduce them into bacteria, fungi, or yeasts to produce recombinant chymosin during fermentation. This fermentation-produced chymosin (FPC) is widely used in industrial cheesemaking in North America and Europe due to its lower cost compared to animal rennet.
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Rennet is added to milk in liquid, paste, powder or tablet form
Rennet is a complex set of enzymes produced in the stomachs of ruminant mammals. It is used in cheese-making to separate milk into solid curds and liquid whey. Rennet is added to milk in liquid, paste, powder, or tablet form.
Liquid rennet is available in animal-based and vegetarian varieties. Animal-based liquid rennet is harder to source than its vegetarian counterpart. It can be purchased from cheese shops, specialty grocers, and cooking stores. Liquid rennet can be stored in a sealed container in the refrigerator for almost a year. On the other hand, vegetarian liquid rennet, also known as microbial rennet, is typically a double-strength liquid and should be used in half the amount specified in recipes. It is created in a lab and is sometimes derived from mushrooms.
Rennet paste is another form in which rennet is added to milk. It is available in animal-based and vegetable-based varieties. The animal-based variety is harder to source than the vegetable-based one. Vegetable rennet paste does not last as long as the animal-based variety.
Powdered rennet is also used in modern cheesemaking practices. It is available in both animal-based and vegetable-based varieties.
Tablet rennet is the easiest form of rennet to find. It is available in powder, liquid, and paste forms. Tablets of rennet can be frozen and stored in an airtight container for up to three years.
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Rennet is crucial for finding the right texture for your cheese
Rennet is a complex set of enzymes produced in the stomachs of ruminant mammals. It is a key ingredient in cheesemaking, used to separate milk into solid curds and liquid whey. This process is crucial for finding the right texture for your cheese.
The enzymes in rennet act on the proteins in milk, causing them to clump together and form a solid mass. The curds are then raked and cut into slabs, while the whey is drained off. The formation of firm curds is essential for creating a cheese with the desired texture. Different types of cheese require different levels of firmness in the curds. For example, a soft Brie requires loose curds, while a hard Romano benefits from firmer curds.
The main enzyme in rennet is chymosin, which is responsible for coagulation. Another important enzyme present in rennet is pepsin, which impacts the aging process of cheese. The ratio of chymosin to pepsin in rennet affects the yield, flavour profile, and texture of the cheese. Too much pepsin can adversely affect the texture of the cheese.
There are different sources of rennet, including animal, vegetable, and microbial rennet. Animal rennet is traditionally sourced from the stomachs of young ruminants, such as calves, lambs, and goats. Vegetable rennet is derived from plants that naturally produce the chymosin enzyme, such as cardoon thistle, artichokes, and nettles. Microbial rennet, also known as vegetarian rennet, is created in a lab and is typically 100% chymosin.
The choice of rennet source can impact the texture and flavour of the cheese. Animal-derived rennet is generally better for aged cheeses as it lasts longer, while vegetable rennet can add bitterness if aged for too long. The type of rennet and the specific enzymes it contains will determine the final texture and quality of the cheese.
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Rennet is used to make vegetarian and vegan cheese
Rennet is a set of enzymes produced in the stomachs of ruminant mammals. It is used in cheesemaking to separate milk into solid curds and liquid whey. Rennet contains the enzyme chymosin, which curdles the casein in milk. While traditional rennet is derived from animal organs, there are now vegetarian and vegan alternatives available.
Vegetarian rennet, also known as microbial rennet, is produced by microorganisms such as fungi, yeast, or mold. It is often referred to as fermentation-produced chymosin (FPC). Microbial rennet is created by genetically modifying microbes with the same genes that produce enzymes in the stomach of a ruminant animal. This results in microorganisms that produce rennet that is similar to animal rennet but suitable for vegetarians. Over 90% of cheeses made commercially today are produced using FPC, as it is less expensive than animal rennet.
Vegetable rennet, derived from plants such as cardoon thistle, artichokes, and nettles, is another alternative for vegetarians. However, vegetable rennet may lead to bitter flavours in the cheese if aged for too long. In addition, it has a shorter shelf life compared to animal rennet. Some traditional cheese production in the Mediterranean uses enzymes from thistle or Cynara (artichokes and cardoons).
Vegan cheese can be produced using microbial rennet, provided that no animal-based ingredients are used in its production. This makes microbial rennet a versatile option for both vegetarians and vegans.
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Frequently asked questions
Rennet is a complex set of enzymes produced in the stomachs of ruminant mammals.
Rennet contains an enzyme called chymosin, which curdles the casein in milk. Rennet is added to milk to trigger coagulation, separating the milk into solid curds and liquid whey.
There are two main varieties of natural rennet: animal rennet and vegetable rennet. Animal rennet is derived from the stomach lining of young ruminants, while vegetable rennet is sourced from plants that naturally produce the chymosin enzyme.
Rennet is used in cheesemaking to facilitate the formation of firm curds, which are crucial for achieving the desired texture in cheese. It helps extract a higher yield of milk solids compared to other methods.
Yes, there are alternatives to using rennet in cheesemaking. Some cheeses use acids, such as vinegar, or cultures to coagulate the milk instead of rennet. Other methods of coagulation include using plant sources like thistle, cardoon, or microbial sources.

























