
Mozzarella is a soft cheese that is a staple in many households. It is often used in pizzas or grilled cheese sandwiches. While it is a soft cheese, it is not the easiest to make at home. Rennet is a staple ingredient in cheese-making, acting as a coagulant that helps milk to solidify. Rennet is usually produced in the stomachs of ruminant mammals, such as sheep. However, not all cheese is made with animal rennet, and some use microbial or vegetable rennet. So, is mozzarella made with rennet?
| Characteristics | Values |
|---|---|
| Mozzarella made with rennet | Traditional mozzarella is made with rennet |
| Mozzarella made without rennet | Some mozzarella is made without rennet, using an acid coagulant like lemon juice, vinegar, or whey |
| Rennet type | Rennet can be animal-based, microbial, chymosin, chymax, or plant-based |
| Vegetarian mozzarella | Some mozzarella is made with microbial rennet, which is suitable for vegetarians |
| Kosher mozzarella | Kosher mozzarella will not contain animal rennet |
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What You'll Learn

Rennet is required to make mozzarella
Mozzarella cheese is typically made with water buffalo milk, rennet, natural whey starter/starter culture, and salt. If it is made with cow's milk, it is called Fior di latte. Rennet is derived from the stomach lining of ruminant animals, usually a young calf, and is considered a traditional ingredient in cheese-making.
There are different types of rennet used in cheese-making, including animal-based, microbial, chymosin, and plant-based. Microbial rennet, for example, is an enzyme harvested from the fermentation of a fungus called Rhizomucor miehei. GMO microbial rennet is identical to animal-derived rennet but is stronger and less expensive.
For those seeking vegetarian cheese options, it is important to note that not all cheese companies indicate the type of rennet used on the label. However, some brands clearly state their use of non-animal rennet, such as microbial rennet, which is suitable for vegetarians. Kosher cheese, for instance, will never contain animal rennet due to Jewish religious rulings that prohibit the consumption of milk and meat products together.
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Rennet is derived from the stomach lining of ruminant animals
Rennet is a set of enzymes that are usually produced in the stomachs of ruminant mammals, such as sheep and cows. It is considered a traditional rennet as it has been used for the longest time and is an integral part of producing many classic old-world cheeses. Rennet is derived specifically from the fourth lining of the stomach of ruminant animals, often a young calf. This process technically falls into the animal by-product category, which may be uncomfortable for some vegetarians.
Animal rennet is considered a traditional rennet since it has been used for centuries and is essential for producing many classic cheeses. For example, Parmigiano Reggiano and many English Cheddars are made using animal rennet. However, due to the Jewish religious ruling that milk and meat products cannot be consumed together, certified kosher cheese will never contain animal rennet and is always vegetarian.
Today, there are several artisanal cheese companies that make cheese with a non-animal-based rennet. For example, microbial rennet is an enzyme harvested from the fermentation of a fungus called Rhizomucor miehei. GMO microbial rennet is identical to animal-derived rennet, completely natural, and inexpensive. However, it is usually stronger than animal-derived rennet, so directions must be followed carefully. Vegetable rennet, on the other hand, is not recommended unless one knows exactly what they are doing, as it will taste and act differently.
Mozzarella cheese is traditionally made with rennet, specifically from water buffalo milk, though cow's milk can also be used. However, softer cheeses like mozzarella, paneer, ricotta, and cream cheese often use a different coagulant, such as lemon juice, vinegar, or whey, instead of rennet, making them safe for vegetarians. Therefore, while rennet is derived from the stomach lining of ruminant animals and is commonly used in cheese-making, it is not always necessary, and vegetarian alternatives are available.
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Rennet is a coagulant that helps milk to solidify
Rennet is a crucial ingredient in the process of making cheese as it serves as a coagulant, helping milk to solidify. It causes proteins in the milk to web together, which starts the process of separating the milk solids (protein, fat, sugar) from the milk liquid (water). While rennet is not the only coagulant that can be used to make cheese, it is particularly important for creating harder cheeses.
Mozzarella is a soft cheese, and softer styles of cheese like mozzarella, paneer, ricotta, and cream cheese often use an acid, such as lemon juice, vinegar, or whey, as their coagulant instead of rennet. These softer cheeses are often safe for vegetarians, as they do not contain rennet, which is an animal byproduct. Rennet is derived from the stomach lining of ruminant animals, usually a young calf, or, in the case of traditional Parmesan, always from an animal source.
However, some traditional cheeses, such as mozzarella, do use rennet. Traditional mozzarella is made from water buffalo milk, rennet, natural whey starter/starter culture, and salt. If it is made from cow's milk, it is called Fior di latte. Rennet is required to give the connections between the protein bundles enough strength to create a cheese with a texture like mozzarella.
There are also vegetarian alternatives to animal-based rennet, such as microbial rennet, which is an enzyme harvested from the fermentation of a fungus called Rhizomucor miehei. GMO microbial rennet is identical to animal-derived rennet but is stronger and often less expensive.
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Rennet alternatives include lemon juice, vinegar, or whey
Rennet is an enzyme that curdles milk, separating curds from whey, and is essential in the process of making cheese. It is typically sourced from the stomach of ruminants, such as cows, sheep, and goats. However, for those seeking non-animal-based alternatives, rennet can be substituted with lemon juice, vinegar, or whey.
Lemon juice, vinegar, and whey are effective replacements for rennet in the cheese-making process. They serve the same function as rennet by curdling milk and facilitating the separation of curds and whey. This separation is crucial for forming the solid structure of cheese. While rennet acts slowly to curdle the milk, these alternatives work more rapidly, achieving the same outcome.
Lemon juice, in particular, can be used in place of citric acid, which is commonly used in cheese-making. However, some have cautioned against relying solely on lemon juice for more complex cheeses like mozzarella, as it may not yield the desired results. Combining lemon juice with other alternatives or "vegan rennet" can lead to better flavour and texture.
Vinegar is another viable option for cheese-making without rennet. It can be used to create a tangy, melty, and slightly stretchy cheese. Similar to lemon juice, combining vinegar with other alternatives, such as "vegan rennet," can improve the overall flavour and texture of the final product.
Whey, the liquid byproduct of cheese-making, can also be utilised as a rennet alternative. By allowing the curds to sit in the cooled whey, the cheese can retain its shape and form. This method helps avoid the rubbery texture that can result from using ice water.
While these alternatives can create cheese without rennet, it is important to note that achieving the flavour and texture of aged cheeses, like traditional mozzarella, typically requires the use of rennet. The strength and slow curdling action of rennet are key to developing the unique characteristics of these cheeses.
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Rennet-free mozzarella is possible but is not authentic
Mozzarella is a soft cheese, and softer cheeses like mozzarella, cottage cheese, cream cheese, ricotta, and goat cheese are often not made with rennet. Instead, they use a coagulant such as lemon juice, vinegar, or whey. However, traditional mozzarella is made with rennet, and some sources argue that rennet-free mozzarella is not authentic.
Rennet is a set of enzymes usually produced in the stomachs of ruminant mammals, such as sheep. It is a traditional ingredient in cheesemaking because it serves as a coagulant, helping milk to solidify. The process of making traditional mozzarella involves using water buffalo milk, rennet, natural whey starter/starter culture, and salt. This type of mozzarella is considered more authentic and has a specific texture that is difficult to replicate without using rennet.
While it is possible to make mozzarella without rennet, some people argue that it is not true mozzarella. One source states that while there are recipes available that only require milk and white vinegar, these are not for making mozzarella but rather "a fake hacked version which is inferior and nothing like real Mozzarella." Another source agrees, stating that while it is possible to make a melty, slightly stretchy cheese without rennet, it is "not technically mozz."
However, it is important to note that not all cheese companies will indicate what type of rennet they use on the label. Some cheeses with animal rennet may simply list “rennet,” “enzymes," or “animal enzymes” on the ingredients list without specifying that they are animal-based. Therefore, for those seeking vegetarian options, it may be necessary to do additional research or shop at a specialty cheese shop where a cheesemonger can provide more detailed information about the products they sell.
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Frequently asked questions
Mozzarella cheese can be made with or without rennet. Traditional mozzarella is made from water buffalo milk, rennet, natural whey starter/starter culture, and salt. However, softer styles of mozzarella use whey or another type of acid (e.g. lemon juice or vinegar) as a coagulant instead of rennet, making it safe for vegetarians.
Rennet is a set of enzymes usually produced in the stomachs of ruminant mammals, such as cows and sheep. It is considered a traditional ingredient in cheesemaking, serving as a coagulant that helps milk to solidify.
Alternatives to animal rennet include microbial rennet and plant-based rennet. Microbial rennet is an enzyme harvested from the fermentation of a fungus called Rhizomucor miehei.
To determine if mozzarella cheese contains rennet, look at the ingredients list on the packaging. Cheeses with animal rennet will typically include terms like "rennet", "animal enzymes", or simply "enzymes". Many cheese brands that use microbial rennet will also indicate this on the label. If the cheese is labelled as suitable for vegetarians, it is likely free of animal rennet.
Yes, it is possible to make homemade mozzarella without rennet. Some recipes use milk (not ultra-pasteurized) and white vinegar to create a melty, slightly stretchy cheese. However, it will not have the same texture as traditional mozzarella, which requires rennet to form connections between protein bundles for a chewier texture.

























